ABSTRACT Background: Children and adolescents with at-risk genotypes (AA/AT) of the rs9939609 polymorphism in FTO, a fat mass and obesity-associated gene, may exhibit different cardiometabolic profile responses than subjects with the TT genotype after an interdisciplinary intervention. Methods: The sample consisted of 36 school children from southern Brazil. We used DNA quantitation and real-time polymerase chain reaction (PCR) for polymorphism genotyping. We measured anthropometric parameters (body mass index (BMI), waist circumference, hip circumference, waist-hip ratio, body fat percentage and skinfold sum), biochemical parameters (glucose, lipid profile, ultra-sensitive C-reactive protein, uric acid, alanine aminotransferase, aspartate aminotransferase, insulin and adiponectin) and blood pressure. The 4-month intervention consisted of physical education classes, nutritional counseling, and postural and oral health counseling. Results: We observed no significant differences among the groups (AA, AT and TT) after the intervention. However, we observed improvements in three parameters (waist circumference, hip circumference and C-reactive protein) in the AT/AA genotype group and in two parameters (hip circumference and uric acid) in the TT genotype group. Conclusions: After an intervention program, carriers of at-risk genotypes for obesity (AA/AT) do not exhibit differences in biochemical parameters, blood pressure and anthropometric parameters compared with carriers of the TT genotype.

A ativação da inflamação do tecido adiposo está relacionada com a obesidade, causada pelo acúmulo de lipídios nos adipócitos. Através dessa ativação, citocinas pró-inflamatórias como Interleucina-6 (IL-6) e Proteína C-Reativa (CRP) parecem influenciar as desordens metabólicas. O presente estudo avaliou se polimorfis mos nos genes da CRP (rs1205) e da IL-6(rs1800795 rs2069845) estão relacionados com o desenvolvimento de alterações metabólicas em crianças e adolescentes. Estudo transversal, foi feito com 470 escolares do município de Santa Cruz do Sul, Brasil, com idades entre 7 a 17 anos. O índice de massa corporal (IMC) foi classificado de acordo com sobrepeso e obesidade. A genotipagem foi realizada através da Reação em Cadeia de Polimerase (PCR) em tempo real. Características antropométricas, indicadores bioquímicos, marcadores imunológicos e a pressão arterial foram avaliados. Estatísticas descritivas, qui-quadrado e regressão logística foram utilizadas para as análises. Nenhuma associação foi detectada entre o polimorfismo rs1800795 e as variáveis avaliadas. Indivíduos com o genótipo de risco no gene rs1205 foram associados ao risco de desenvolver hipercolesterolemia (OR = 2,79; IC: 1,40, 5,57; p = 0,003). Portadores do genótipo de risco no gene rs2069845 estão associados com o risco de desenvolver obesidade (OR = 3,07; IC: 1,08, 8,72; p = 0,03). O polimorfismo rs2069845 foi relacionado com obesidade e o rs1205 associado ao risco de desen volver hipercolesterolemia em escolares brasileiros.

Activation of adipose tissue inflammation is associated with obesity caused by lipid accumulation in adipocytes. Through this activation, proinflammatory cytokines, such as Interleukin-6 (IL-6) and C-reactive protein (CRP) seem to influence metabolic disorders. The present study evaluated whether polymorphisms in the CRP (rs1205) and IL-6 (rs1800795, rs2069845) genes are associated with the development of metabolic disorders in children and adolescents. A cross-sectional study was performed, consisting of 470 students from the municipality of Santa Cruz do Sul, Brazil, aged 7-17 years. Body mass index (BMI) was classified according to overweight and obesity. Genotyping was performed by real-time Polymerase Chain Reaction(PCR). Anthropometric characteristics, biochemical markers, immunological markers and blood pressure were assessed. Descriptive statistics, chi-square and logistic regression were used for the analyses. No association was detected between the rs1800795 polymorphism and the assessed variables. Individuals with the risk genotype in the rs1205 gene were associated with the risk of developing hypercholesterolemia (OR 2.79; CI 1.40, 5.57; p = 0.003). Carriers of the risk genotype in the rs2069845 gene are associated with the risk of developing obesity (OR 3.07; CI 1.08, 8.72; p = 0.03). The polymorphism rs2069845 was associated with obesity and rs1205 was associated with the risk of developing hypercholesterolemia in Brazilian schoolchildren.